Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1137
intra-residue [i = j]168
sequential [| i - j | = 1]337
medium range [1 < | i - j | < 5]217
long range [| i - j | ≥ 5]415
NOE constraints per restrained residue b13.1
Hydrogen bond constraints:
Total54
long range [| i - j | ≥ 5]32
Dihedral-angle constraints:179
Total number of restricting constraints b1370
Total number of restricting constraints per restrained residue b15.7
Restricting long-range constraints per restrained residue b5.1
Total structures computedcurrently unknown
Number of structures used10
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å12.6
0.2 - 0.5 Å20.4
> 0.5 Å69.8
RMS of distance violation / constraint1.56 Å
Maximum distance violation d12.43 Å
Dihedral angle violations / structure
1 - 10 °6.4
> 10 °6.5
RMS of dihedral angle violation / constraint3.72 °
Maximum dihedral angle violation d45.40 °
RPF scores
RecallPrecisionF-measureDP-score
0.890.7870.8350.723
RMSD Values
allorderedeSelectedf
All backbone atoms2.8 Å1.2 Å1.2 Å
All heavy atoms3.6 Å1.7 Å1.7 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.28N/A-0.79
Procheck G-factor e (all dihedral angles)0.07N/A0.41
Verify3D0.350.0465-1.77
ProsaII (-ve)0.570.0499-0.33
MolProbity clashscore7.452.66910.25
General linear model RMSD prediction1.58
Ramachandran Plot Summary from Procheck f
Most favoured regions88.8%
Additionally allowed regions9.2%
Generously allowed regions0.6%
Disallowed regions1.5%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions95.9%
Allowed regions3.9%
Disallowed regions0.2%


a Analysed for residues 1 to 91
b There are 87 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 14A-18A,28A-32A,38A-50A,53A-68A,71A-82A
f Residues selected based on: User defined residues

Selected residue ranges: 14A-18A,28A-32A,38A-50A,53A-67A,70A-82A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4