Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1137
intra-residue [i = j]168
sequential [| i - j | = 1]337
medium range [1 < | i - j | < 5]217
long range [| i - j | ≥ 5]415
NOE constraints per restrained residue b13.1
Hydrogen bond constraints:
Total54
long range [| i - j | ≥ 5]32
Dihedral-angle constraints:179
Total number of restricting constraints b1370
Total number of restricting constraints per restrained residue b15.7
Restricting long-range constraints per restrained residue b5.1
Total structures computedcurrently unknown
Number of structures used10
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å7.5
0.2 - 0.5 Å3.1
> 0.5 Å2.1
RMS of distance violation / constraint0.03 Å
Maximum distance violation d1.09 Å
Dihedral angle violations / structure
1 - 10 °1.2
> 10 °0.4
RMS of dihedral angle violation / constraint1.45 °
Maximum dihedral angle violation d50.70 °
RPF scores
RecallPrecisionF-measureDP-score
0.9280.7810.8480.764
RMSD Values
allorderedeSelectedf
All backbone atoms2.7 Å0.6 Å0.6 Å
All heavy atoms3.4 Å0.8 Å0.8 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.11N/A-0.12
Procheck G-factor e (all dihedral angles)0.11N/A0.65
Verify3D0.360.0397-1.61
ProsaII (-ve)0.550.0837-0.41
MolProbity clashscore9.962.7661-0.18
General linear model RMSD prediction1.29
Ramachandran Plot Summary from Procheck f
Most favoured regions90.8%
Additionally allowed regions7.1%
Generously allowed regions1.2%
Disallowed regions0.8%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions95.5%
Allowed regions4.3%
Disallowed regions0.2%


a Analysed for residues 1 to 91
b There are 87 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 14A-18A,28A-32A,38A-50A,53A-68A,70A-82A
f Residues selected based on: User defined residues

Selected residue ranges: 14A-18A,28A-32A,38A-50A,53A-67A,70A-82A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4