Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1540
intra-residue [i = j]324
sequential [| i - j | = 1]413
medium range [1 < | i - j | < 5]321
long range [| i - j | ≥ 5]482
NOE constraints per restrained residue b19.7
Dihedral-angle constraints:231
Total number of restricting constraints b1771
Total number of restricting constraints per restrained residue b22.7
Restricting long-range constraints per restrained residue b6.2
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å12.2
0.2 - 0.5 Å4.7
> 0.5 Å1.65
RMS of distance violation / constraint0.01 Å
Maximum distance violation d0.76 Å
Dihedral angle violations / structure
1 - 10 °1.5
> 10 °2
RMS of dihedral angle violation / constraint1.35 °
Maximum dihedral angle violation d20.30 °
RPF scores
RecallPrecisionF-measureDP-score
0.9620.9160.9390.796
RMSD Values
allorderedeSelectedf
All backbone atoms1.3 Å0.4 Å0.4 Å
All heavy atoms1.6 Å0.6 Å0.6 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.04N/A0.47
Procheck G-factor e (all dihedral angles)0.13N/A0.77
Verify3D0.390.0313-1.12
ProsaII (-ve)0.610.0971-0.17
MolProbity clashscore15.862.1275-1.20
General linear model RMSD prediction0.91
Ramachandran Plot Summary from Procheck f
Most favoured regions93.9%
Additionally allowed regions6.1%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions95.4%
Allowed regions4.6%
Disallowed regions0%


a Analysed for residues 1 to 78
b There are 78 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 3A-7A,13A-34A,37A-55A,58A-74A
f Residues selected based on: User defined residues

Selected residue ranges: 3A-7A,13A-34A,38A-55A,58A-74A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4