Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2064
intra-residue [i = j]388
sequential [| i - j | = 1]611
medium range [1 < | i - j | < 5]440
long range [| i - j | ≥ 5]625
NOE constraints per restrained residue b17.8
Dihedral-angle constraints:128
Total number of restricting constraints b2192
Total number of restricting constraints per restrained residue b18.9
Restricting long-range constraints per restrained residue b5.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å17.75
0.2 - 0.5 Å40.65
> 0.5 Å139.7
RMS of distance violation / constraint0.67 Å
Maximum distance violation d7.79 Å
Dihedral angle violations / structure
1 - 10 °11.2
> 10 °5.95
RMS of dihedral angle violation / constraint4.57 °
Maximum dihedral angle violation d51.00 °
RPF scores
RecallPrecisionF-measureDP-score
0.8760.9380.9060.707
RMSD Values
allorderedeSelectedf
All backbone atoms2.1 Å1.2 Å1.2 Å
All heavy atoms2.7 Å1.7 Å1.7 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.32N/A-0.94
Procheck G-factor e (all dihedral angles)0.07N/A0.41
Verify3D0.320.0284-2.25
ProsaII (-ve)0.470.0618-0.74
MolProbity clashscore3.381.13410.95
General linear model RMSD prediction1.98
Ramachandran Plot Summary from Procheck f
Most favoured regions87.3%
Additionally allowed regions12.7%
Generously allowed regions0.1%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions97.9%
Allowed regions2%
Disallowed regions0.1%


a Analysed for residues 1 to 121
b There are 116 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 6A-56A,60A-70A,74A-108A,110A-114A
f Residues selected based on: User defined residues

Selected residue ranges: 6A-55A,60A-70A,74A-108A,110A-114A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4