Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2064
intra-residue [i = j]388
sequential [| i - j | = 1]611
medium range [1 < | i - j | < 5]440
long range [| i - j | ≥ 5]625
NOE constraints per restrained residue b17.8
Dihedral-angle constraints:128
Total number of restricting constraints b2192
Total number of restricting constraints per restrained residue b18.9
Restricting long-range constraints per restrained residue b5.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å0.9
0.2 - 0.5 Å0.1
> 0.5 Å0
RMS of distance violation / constraint0.00 Å
Maximum distance violation d0.24 Å
Dihedral angle violations / structure
1 - 10 °3.6
> 10 °0
RMS of dihedral angle violation / constraint0.32 °
Maximum dihedral angle violation d3.30 °
RPF scores
RecallPrecisionF-measureDP-score
0.9110.930.920.753
RMSD Values
allorderedeSelectedf
All backbone atoms1.6 Å0.7 Å0.7 Å
All heavy atoms2.1 Å1.1 Å1.1 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.42N/A-1.34
Procheck G-factor e (all dihedral angles)-0.40N/A-2.37
Verify3D0.260.0192-3.21
ProsaII (-ve)0.430.0577-0.91
MolProbity clashscore22.042.5878-2.26
General linear model RMSD prediction1.97
Ramachandran Plot Summary from Procheck f
Most favoured regions85.1%
Additionally allowed regions14.5%
Generously allowed regions0.4%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions94.2%
Allowed regions5%
Disallowed regions0.8%


a Analysed for residues 1 to 121
b There are 116 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 6A-55A,60A-70A,74A-108A,110A-114A
f Residues selected based on: User defined residues

Selected residue ranges: 6A-55A,60A-70A,74A-108A,110A-114A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4