Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1513
intra-residue [i = j]373
sequential [| i - j | = 1]424
medium range [1 < | i - j | < 5]206
long range [| i - j | ≥ 5]510
NOE constraints per restrained residue b11.2
Dihedral-angle constraints:660
Total number of restricting constraints b2173
Total number of restricting constraints per restrained residue b16.1
Restricting long-range constraints per restrained residue b3.8
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å18.05
0.2 - 0.5 Å29.85
> 0.5 Å75.7
RMS of distance violation / constraint0.76 Å
Maximum distance violation d9.96 Å
Dihedral angle violations / structure
1 - 10 °21.55
> 10 °11.2
RMS of dihedral angle violation / constraint2.68 °
Maximum dihedral angle violation d81.00 °
RMSD Values
allorderedeSelectedf
All backbone atoms5.4 Å1.0 Å1.0 Å
All heavy atoms6.1 Å1.4 Å1.4 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.01N/A0.35
Procheck G-factor e (all dihedral angles)0.20N/A1.18
Verify3D0.420.0261-0.64
ProsaII (-ve)0.540.0441-0.45
MolProbity clashscore4.421.11850.77
Ramachandran Plot Summary from Procheck f
Most favoured regions93.8%
Additionally allowed regions6.1%
Generously allowed regions0.1%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.9%
Allowed regions1.1%
Disallowed regions0%


a Analysed for residues 1 to 142
b There are 135 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 9A-17A,20A-41A,58A-65A,67A-74A,78A-88A,93A-106A,115A-125A
f Residues selected based on: User defined residues

Selected residue ranges: 9A-17A,20A-41A,58A-74A,78A-88A,93A-106A,115A-125A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4