Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1226
intra-residue [i = j]263
sequential [| i - j | = 1]374
medium range [1 < | i - j | < 5]324
long range [| i - j | ≥ 5]265
NOE constraints per restrained residue b11.2
Hydrogen bond constraints:
Total84
long range [| i - j | ≥ 5]0
Dihedral-angle constraints:128
Total number of restricting constraints b1438
Total number of restricting constraints per restrained residue b13.2
Restricting long-range constraints per restrained residue b2.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å2.3
0.2 - 0.5 Å0.6
> 0.5 Å0
RMS of distance violation / constraint0.01 Å
Maximum distance violation d0.34 Å
Dihedral angle violations / structure
1 - 10 °0.3
> 10 °0
RMS of dihedral angle violation / constraint0.09 °
Maximum dihedral angle violation d1.60 °
RPF scores
RecallPrecisionF-measureDP-score
0.98060.8991070.9380.8
RMSD Values
allorderedeSelectedf
All backbone atoms2.7 Å0.8 Å0.8 Å
All heavy atoms3.2 Å1.2 Å1.2 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.36N/A1.73
Procheck G-factor e (all dihedral angles)0.19N/A1.12
Verify3D0.300.0295-2.57
ProsaII (-ve)0.670.07740.08
MolProbity clashscore19.932.3860-1.89
General linear model RMSD prediction0.93
Ramachandran Plot Summary from Procheck f
Most favoured regions96.1%
Additionally allowed regions3.9%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions96.1%
Allowed regions3.9%
Disallowed regions0%


a Analysed for residues 1 to 109
b There are 109 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 11A-19A,31A-63A,67A-83A,95A-108A
f Residues selected based on: User defined residues

Selected residue ranges: 11A-19A,31A-63A,67A-83A,95A-108A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4