Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1661
intra-residue [i = j]542
sequential [| i - j | = 1]444
medium range [1 < | i - j | < 5]362
long range [| i - j | ≥ 5]313
NOE constraints per restrained residue b13.4
Dihedral-angle constraints:273
Total number of restricting constraints b1934
Total number of restricting constraints per restrained residue b15.6
Restricting long-range constraints per restrained residue b2.5
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å25.8
0.2 - 0.5 Å38
> 0.5 Å35.05
RMS of distance violation / constraint0.55 Å
Maximum distance violation d7.40 Å
Dihedral angle violations / structure
1 - 10 °12
> 10 °2.75
RMS of dihedral angle violation / constraint2.22 °
Maximum dihedral angle violation d46.60 °
RPF scores
RecallPrecisionF-measureDP-score
0.9840.8670.9220.708
RMSD Values
allorderedeSelectedf
All backbone atoms1.8 Å0.7 Å0.7 Å
All heavy atoms2.4 Å1.1 Å1.1 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.58N/A2.60
Procheck G-factor e (all dihedral angles)0.62N/A3.67
Verify3D0.350.0248-1.77
ProsaII (-ve)0.900.06021.03
MolProbity clashscore2.690.95681.06
General linear model RMSD prediction1.19
Ramachandran Plot Summary from Procheck f
Most favoured regions97.5%
Additionally allowed regions2.5%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.7%
Allowed regions0.3%
Disallowed regions0.1%


a Analysed for residues 1 to 129
b There are 124 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 6A-56A,63A-70A,73A-91A,100A-122A
f Residues selected based on: User defined residues

Selected residue ranges: 6A-45A,47A-56A,63A-70A,73A-91A,100A-122A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4