Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total870
intra-residue [i = j]188
sequential [| i - j | = 1]232
medium range [1 < | i - j | < 5]271
long range [| i - j | ≥ 5]179
NOE constraints per restrained residue b11.9
Dihedral-angle constraints:96
Total number of restricting constraints b966
Total number of restricting constraints per restrained residue b13.2
Restricting long-range constraints per restrained residue b2.5
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å16.6
0.2 - 0.5 Å16.3
> 0.5 Å2.5
RMS of distance violation / constraint0.08 Å
Maximum distance violation d2.39 Å
Dihedral angle violations / structure
1 - 10 °0.3
> 10 °0
RMS of dihedral angle violation / constraint0.21 °
Maximum dihedral angle violation d8.10 °
RPF scores
RecallPrecisionF-measureDP-score
0.7480.6440.6920.585
RMSD Values
allorderedeSelectedf
All backbone atoms4.8 Å2.9 Å3.4 Å
All heavy atoms5.4 Å3.2 Å3.8 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.54N/A2.44
Procheck G-factor e (all dihedral angles)0.42N/A2.48
Verify3D0.300.0304-2.57
ProsaII (-ve)0.300.0832-1.45
MolProbity clashscore15.632.9918-1.16
General linear model RMSD prediction2.01
Ramachandran Plot Summary from Procheck f
Most favoured regions90.9%
Additionally allowed regions6.9%
Generously allowed regions2.2%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions93.3%
Allowed regions5.1%
Disallowed regions1.6%


a Analysed for residues 1 to 170
b There are 73 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 7A-19A,24A-43A,47A-69A,7B-19B,24B-43B,47B-69B
f Residues selected based on: User defined residues

Selected residue ranges: 7A-20A,24A-42A,47A-69A,7B-19B,22B-42B,47B-68B

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4