Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1023
intra-residue [i = j]306
sequential [| i - j | = 1]207
medium range [1 < | i - j | < 5]318
long range [| i - j | ≥ 5]192
NOE constraints per restrained residue b23.2
Hydrogen bond constraints:
Total48
long range [| i - j | ≥ 5]0
Dihedral-angle constraints:68
Total number of restricting constraints b1139
Total number of restricting constraints per restrained residue b25.9
Restricting long-range constraints per restrained residue b4.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å2.55
0.2 - 0.5 Å6.1
> 0.5 Å15.55
RMS of distance violation / constraint0.22 Å
Maximum distance violation d3.24 Å
Dihedral angle violations / structure
1 - 10 °0.55
> 10 °0.1
RMS of dihedral angle violation / constraint0.87 °
Maximum dihedral angle violation d21.00 °
RPF scores
RecallPrecisionF-measureDP-score
0.9920.9450.9680.827
RMSD Values
allorderedeSelectedf
All backbone atoms3.4 Å0.2 Å0.2 Å
All heavy atoms3.9 Å0.6 Å0.6 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.65N/A2.87
Procheck G-factor e (all dihedral angles)0.69N/A4.08
Verify3D0.290.0294-2.73
ProsaII (-ve)0.980.09301.36
MolProbity clashscore0.760.95751.40
General linear model RMSD prediction-0.14
Ramachandran Plot Summary from Procheck f
Most favoured regions99.3%
Additionally allowed regions0.7%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions100%
Allowed regions0%
Disallowed regions0%


a Analysed for residues 1 to 48
b There are 44 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 3A-38A
f Residues selected based on: User defined residues

Selected residue ranges: 2A-38A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4