Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2023
intra-residue [i = j]419
sequential [| i - j | = 1]498
medium range [1 < | i - j | < 5]626
long range [| i - j | ≥ 5]480
NOE constraints per restrained residue b18.6
Hydrogen bond constraints:
Total108
long range [| i - j | ≥ 5]0
Dihedral-angle constraints:175
Total number of restricting constraints b2306
Total number of restricting constraints per restrained residue b21.2
Restricting long-range constraints per restrained residue b4.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å18.7
0.2 - 0.5 Å33.75
> 0.5 Å44.1
RMS of distance violation / constraint0.28 Å
Maximum distance violation d7.35 Å
Dihedral angle violations / structure
1 - 10 °7.3
> 10 °3.1
RMS of dihedral angle violation / constraint2.75 °
Maximum dihedral angle violation d48.40 °
RPF scores
RecallPrecisionF-measureDP-score
0.9550.8910.9220.729
RMSD Values
allorderedeSelectedf
All backbone atoms1.9 Å0.7 Å0.7 Å
All heavy atoms2.4 Å1.2 Å1.2 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.61N/A2.71
Procheck G-factor e (all dihedral angles)0.65N/A3.84
Verify3D0.410.0225-0.80
ProsaII (-ve)0.990.04041.41
MolProbity clashscore4.191.56930.81
General linear model RMSD prediction1.04
Ramachandran Plot Summary from Procheck f
Most favoured regions96.6%
Additionally allowed regions3.3%
Generously allowed regions0.1%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.1%
Allowed regions0.8%
Disallowed regions0.1%


a Analysed for residues 1 to 123
b There are 109 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 7A-26A,42A-64A,70A-92A,97A-120A
f Residues selected based on: User defined residues

Selected residue ranges: 7A-26A,42A-64A,70A-93A,97A-120A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4