Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2023
intra-residue [i = j]419
sequential [| i - j | = 1]498
medium range [1 < | i - j | < 5]626
long range [| i - j | ≥ 5]480
NOE constraints per restrained residue b18.6
Hydrogen bond constraints:
Total108
long range [| i - j | ≥ 5]0
Dihedral-angle constraints:175
Total number of restricting constraints b2306
Total number of restricting constraints per restrained residue b21.2
Restricting long-range constraints per restrained residue b4.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å12.75
0.2 - 0.5 Å11.25
> 0.5 Å1.65
RMS of distance violation / constraint0.03 Å
Maximum distance violation d1.07 Å
Dihedral angle violations / structure
1 - 10 °0.05
> 10 °0
RMS of dihedral angle violation / constraint0.04 °
Maximum dihedral angle violation d2.50 °
RPF scores
RecallPrecisionF-measureDP-score
0.9640.8920.9260.747
RMSD Values
allorderedeSelectedf
All backbone atoms1.7 Å0.5 Å0.5 Å
All heavy atoms2.2 Å0.9 Å0.9 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.81N/A3.50
Procheck G-factor e (all dihedral angles)0.73N/A4.32
Verify3D0.360.0317-1.61
ProsaII (-ve)0.990.05301.41
MolProbity clashscore5.002.50880.67
General linear model RMSD prediction0.78
Ramachandran Plot Summary from Procheck f
Most favoured regions98.9%
Additionally allowed regions1.1%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.5%
Allowed regions0.4%
Disallowed regions0.1%


a Analysed for residues 1 to 123
b There are 109 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 7A-26A,42A-64A,70A-92A,97A-120A
f Residues selected based on: User defined residues

Selected residue ranges: 7A-26A,42A-64A,70A-93A,97A-120A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4