Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1844
intra-residue [i = j]468
sequential [| i - j | = 1]556
medium range [1 < | i - j | < 5]140
long range [| i - j | ≥ 5]680
NOE constraints per restrained residue b22.5
Hydrogen bond constraints:
Total112
long range [| i - j | ≥ 5]64
Dihedral-angle constraints:172
Total number of restricting constraints b2128
Total number of restricting constraints per restrained residue b26.0
Restricting long-range constraints per restrained residue b9.1
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å7.55
0.2 - 0.5 Å2.2
> 0.5 Å0.4
RMS of distance violation / constraint0.02 Å
Maximum distance violation d0.78 Å
Dihedral angle violations / structure
1 - 10 °1.05
> 10 °0
RMS of dihedral angle violation / constraint0.32 °
Maximum dihedral angle violation d6.10 °
RPF scores
RecallPrecisionF-measureDP-score
0.9690.9090.9380.798
RMSD Values
allorderedeSelectedf
All backbone atoms3.4 Å0.7 Å0.7 Å
All heavy atoms4.0 Å1.0 Å1.0 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.22N/A-0.55
Procheck G-factor e (all dihedral angles)0.04N/A0.24
Verify3D0.320.0355-2.25
ProsaII (-ve)0.260.0862-1.61
MolProbity clashscore8.902.3971-0.00
General linear model RMSD prediction2.04
Ramachandran Plot Summary from Procheck f
Most favoured regions94.0%
Additionally allowed regions5.9%
Generously allowed regions0.1%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99%
Allowed regions0.9%
Disallowed regions0.1%


a Analysed for residues 1 to 174
b There are 82 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 8A-21A,25A-34A,40A-46A,57A-73A,8B-21B,25B-34B,40B-46B,57B-73B
f Residues selected based on: User defined residues

Selected residue ranges: 8A-21A,25A-34A,40A-46A,57A-73A,8B-21B,25B-34B,40B-47B,57B-73B

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4