Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1356
intra-residue [i = j]0
sequential [| i - j | = 1]198
medium range [1 < | i - j | < 5]778
long range [| i - j | ≥ 5]380
NOE constraints per restrained residue b19.4
Hydrogen bond constraints:
Total122
long range [| i - j | ≥ 5]0
Dihedral-angle constraints:251
Total number of restricting constraints b1729
Total number of restricting constraints per restrained residue b24.7
Restricting long-range constraints per restrained residue b5.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å14.35
0.2 - 0.5 Å29.95
> 0.5 Å66
RMS of distance violation / constraint0.25 Å
Maximum distance violation d5.32 Å
Dihedral angle violations / structure
1 - 10 °2.4
> 10 °1.7
RMS of dihedral angle violation / constraint2.05 °
Maximum dihedral angle violation d46.40 °
RPF scores
RecallPrecisionF-measureDP-score
0.8483940.9153490.8810.783132
RMSD Values
allorderedeSelectedf
All backbone atoms3.4 Å0.8 Å0.8 Å
All heavy atoms3.9 Å1.3 Å1.3 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.49N/A2.24
Procheck G-factor e (all dihedral angles)0.56N/A3.31
Verify3D0.380.0303-1.28
ProsaII (-ve)1.010.05111.49
MolProbity clashscore3.231.42840.97
General linear model RMSD prediction1.35
Ramachandran Plot Summary from Procheck f
Most favoured regions97.3%
Additionally allowed regions2.6%
Generously allowed regions0.0%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.5%
Allowed regions0.5%
Disallowed regions0%


a Analysed for residues 1 to 170
b There are 70 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 9A-73A,9B-73B
f Residues selected based on: User defined residues

Selected residue ranges: 9A-73A,9B-73B

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4