Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1790
intra-residue [i = j]497
sequential [| i - j | = 1]465
medium range [1 < | i - j | < 5]399
long range [| i - j | ≥ 5]429
NOE constraints per restrained residue b12.7
Hydrogen bond constraints:
Total116
long range [| i - j | ≥ 5]4
Dihedral-angle constraints:196
Total number of restricting constraints b2102
Total number of restricting constraints per restrained residue b14.9
Restricting long-range constraints per restrained residue b3.1
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å5.7
0.2 - 0.5 Å3.65
> 0.5 Å1.35
RMS of distance violation / constraint0.04 Å
Maximum distance violation d4.31 Å
Dihedral angle violations / structure
1 - 10 °0.55
> 10 °0.4
RMS of dihedral angle violation / constraint0.77 °
Maximum dihedral angle violation d19.70 °
RPF scores
RecallPrecisionF-measureDP-score
0.9720.9320.9520.805
RMSD Values
allorderedeSelectedf
All backbone atoms4.5 Å0.9 Å0.9 Å
All heavy atoms5.1 Å1.3 Å1.3 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.34N/A1.65
Procheck G-factor e (all dihedral angles)0.40N/A2.37
Verify3D0.410.0322-0.80
ProsaII (-ve)0.700.05540.21
MolProbity clashscore8.653.79550.04
General linear model RMSD prediction1.38
Ramachandran Plot Summary from Procheck f
Most favoured regions96.4%
Additionally allowed regions3.6%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.2%
Allowed regions0.8%
Disallowed regions0%


a Analysed for residues 1 to 155
b There are 141 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 14A-18A,20A-38A,48A-78A,82A-99A,102A-143A
f Residues selected based on: User defined residues

Selected residue ranges: 14A-18A,20A-38A,48A-78A,83A-99A,102A-143A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4