Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1011
intra-residue [i = j]81
sequential [| i - j | = 1]220
medium range [1 < | i - j | < 5]239
long range [| i - j | ≥ 5]471
NOE constraints per restrained residue b6.0
Hydrogen bond constraints:
Total92
long range [| i - j | ≥ 5]34
Dihedral-angle constraints:288
Total number of restricting constraints b1391
Total number of restricting constraints per restrained residue b8.3
Restricting long-range constraints per restrained residue b3.0
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å29.75
0.2 - 0.5 Å14.6
> 0.5 Å1.2
RMS of distance violation / constraint0.08 Å
Maximum distance violation d2.20 Å
Dihedral angle violations / structure
1 - 10 °30.1
> 10 °1.75
RMS of dihedral angle violation / constraint1.78 °
Maximum dihedral angle violation d21.90 °
RPF scores
RecallPrecisionF-measureDP-score
0.7060.9490.8090.686
RMSD Values
allorderedeSelectedf
All backbone atoms4.5 Å0.9 Å0.9 Å
All heavy atoms5.2 Å1.3 Å1.3 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.46N/A-1.49
Procheck G-factor e (all dihedral angles)-0.29N/A-1.71
Verify3D0.400.0204-0.96
ProsaII (-ve)0.410.0449-0.99
MolProbity clashscore19.101.8075-1.75
General linear model RMSD prediction3.08
Ramachandran Plot Summary from Procheck f
Most favoured regions88.2%
Additionally allowed regions11.6%
Generously allowed regions0.1%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions94.3%
Allowed regions5.1%
Disallowed regions0.6%


a Analysed for residues 1 to 202
b There are 168 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 21A-36A,42A-82A,84A-139A,147A-175A,184A-195A
f Residues selected based on: User defined residues

Selected residue ranges: 21A-36A,42A-82A,84A-139A,147A-175A,184A-195A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4