Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1792
intra-residue [i = j]395
sequential [| i - j | = 1]431
medium range [1 < | i - j | < 5]486
long range [| i - j | ≥ 5]480
NOE constraints per restrained residue b18.9
Dihedral-angle constraints:165
Total number of restricting constraints b1957
Total number of restricting constraints per restrained residue b20.6
Restricting long-range constraints per restrained residue b5.1
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å1.85
0.2 - 0.5 Å1.25
> 0.5 Å2.25
RMS of distance violation / constraint0.09 Å
Maximum distance violation d2.98 Å
Dihedral angle violations / structure
1 - 10 °0.05
> 10 °1
RMS of dihedral angle violation / constraint1.05 °
Maximum dihedral angle violation d16.70 °
RPF scores
RecallPrecisionF-measureDP-score
0.9560.8980.9260.81
RMSD Values
allorderedeSelectedf
All backbone atoms1.9 Å0.3 Å0.3 Å
All heavy atoms2.4 Å0.5 Å0.5 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.42N/A1.97
Procheck G-factor e (all dihedral angles)0.48N/A2.84
Verify3D0.430.0199-0.48
ProsaII (-ve)0.960.04891.28
MolProbity clashscore4.341.30250.78
General linear model RMSD prediction0.71
Ramachandran Plot Summary from Procheck f
Most favoured regions97.6%
Additionally allowed regions2.4%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.8%
Allowed regions0.2%
Disallowed regions0%


a Analysed for residues 1 to 101
b There are 95 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 2A-94A
f Residues selected based on: User defined residues

Selected residue ranges: 2A-94A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4