Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1823
intra-residue [i = j]419
sequential [| i - j | = 1]481
medium range [1 < | i - j | < 5]370
long range [| i - j | ≥ 5]553
NOE constraints per restrained residue b14.9
Hydrogen bond constraints:
Total80
long range [| i - j | ≥ 5]40
Dihedral-angle constraints:172
Total number of restricting constraints b2075
Total number of restricting constraints per restrained residue b17.0
Restricting long-range constraints per restrained residue b4.9
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å21.3
0.2 - 0.5 Å41.2
> 0.5 Å125.15
RMS of distance violation / constraint0.58 Å
Maximum distance violation d8.61 Å
Dihedral angle violations / structure
1 - 10 °9.75
> 10 °7.1
RMS of dihedral angle violation / constraint4.21 °
Maximum dihedral angle violation d46.20 °
RPF scores
RecallPrecisionF-measureDP-score
0.8840.8450.8640.707
RMSD Values
allorderedeSelectedf
All backbone atoms2.2 Å1.1 Å1.1 Å
All heavy atoms2.9 Å1.6 Å1.6 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.01N/A0.35
Procheck G-factor e (all dihedral angles)0.22N/A1.30
Verify3D0.440.0310-0.32
ProsaII (-ve)0.780.06210.54
MolProbity clashscore4.351.70710.78
General linear model RMSD prediction1.71
Ramachandran Plot Summary from Procheck f
Most favoured regions91.9%
Additionally allowed regions8.1%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.4%
Allowed regions0.5%
Disallowed regions0%


a Analysed for residues 1 to 125
b There are 122 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 2A-18A,20A-36A,40A-53A,57A-83A,90A-99A,102A-117A
f Residues selected based on: User defined residues

Selected residue ranges: 2A-36A,40A-53A,57A-83A,90A-99A,101A-116A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4