Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1823
intra-residue [i = j]419
sequential [| i - j | = 1]481
medium range [1 < | i - j | < 5]370
long range [| i - j | ≥ 5]553
NOE constraints per restrained residue b14.9
Hydrogen bond constraints:
Total80
long range [| i - j | ≥ 5]40
Dihedral-angle constraints:172
Total number of restricting constraints b2075
Total number of restricting constraints per restrained residue b17.0
Restricting long-range constraints per restrained residue b4.9
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å13.55
0.2 - 0.5 Å4.3
> 0.5 Å0
RMS of distance violation / constraint0.02 Å
Maximum distance violation d0.42 Å
Dihedral angle violations / structure
1 - 10 °6.3
> 10 °0
RMS of dihedral angle violation / constraint0.60 °
Maximum dihedral angle violation d7.60 °
RPF scores
RecallPrecisionF-measureDP-score
0.9080.8440.8750.751
RMSD Values
allorderedeSelectedf
All backbone atoms1.7 Å0.7 Å0.7 Å
All heavy atoms2.3 Å1.1 Å1.1 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.01N/A0.35
Procheck G-factor e (all dihedral angles)-0.04N/A-0.24
Verify3D0.400.0237-0.96
ProsaII (-ve)0.630.0517-0.08
MolProbity clashscore24.473.3239-2.67
General linear model RMSD prediction1.61
Ramachandran Plot Summary from Procheck f
Most favoured regions94.0%
Additionally allowed regions5.8%
Generously allowed regions0.2%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.5%
Allowed regions1.2%
Disallowed regions0.3%


a Analysed for residues 1 to 125
b There are 122 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 2A-36A,40A-53A,57A-83A,90A-99A,101A-116A
f Residues selected based on: User defined residues

Selected residue ranges: 2A-36A,40A-53A,57A-83A,90A-99A,101A-116A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4