Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2261
intra-residue [i = j]615
sequential [| i - j | = 1]620
medium range [1 < | i - j | < 5]297
long range [| i - j | ≥ 5]729
NOE constraints per restrained residue b17.0
Dihedral-angle constraints:203
Total number of restricting constraints b2464
Total number of restricting constraints per restrained residue b18.5
Restricting long-range constraints per restrained residue b5.5
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å18.3
0.2 - 0.5 Å39.05
> 0.5 Å97.9
RMS of distance violation / constraint0.49 Å
Maximum distance violation d10.34 Å
Dihedral angle violations / structure
1 - 10 °7.3
> 10 °3.6
RMS of dihedral angle violation / constraint6.88 °
Maximum dihedral angle violation d157.90 °
RPF scores
RecallPrecisionF-measureDP-score
0.960.8880.9230.738
RMSD Values
allorderedeSelectedf
All backbone atoms5.5 Å4.7 Å4.0 Å
All heavy atoms5.8 Å4.7 Å4.0 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.30N/A-0.87
Procheck G-factor e (all dihedral angles)0.03N/A0.18
Verify3D0.370.0208-1.44
ProsaII (-ve)0.470.0462-0.74
MolProbity clashscore3.951.28410.85
General linear model RMSD prediction1.99
Ramachandran Plot Summary from Procheck f
Most favoured regions88.0%
Additionally allowed regions11.9%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions96.9%
Allowed regions3.1%
Disallowed regions0%


a Analysed for residues 1 to 134
b There are 133 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-80A,87A-119A,129A-133A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-80A,86A-119A,129A-132A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4