Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2261
intra-residue [i = j]615
sequential [| i - j | = 1]620
medium range [1 < | i - j | < 5]297
long range [| i - j | ≥ 5]729
NOE constraints per restrained residue b17.0
Dihedral-angle constraints:203
Total number of restricting constraints b2464
Total number of restricting constraints per restrained residue b18.5
Restricting long-range constraints per restrained residue b5.5
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å6.6
0.2 - 0.5 Å3.6
> 0.5 Å2.1
RMS of distance violation / constraint0.03 Å
Maximum distance violation d1.23 Å
Dihedral angle violations / structure
1 - 10 °0.6
> 10 °0.1
RMS of dihedral angle violation / constraint0.35 °
Maximum dihedral angle violation d11.80 °
RPF scores
RecallPrecisionF-measureDP-score
0.9740.8840.9270.753
RMSD Values
allorderedeSelectedf
All backbone atoms5.4 Å4.7 Å4.0 Å
All heavy atoms5.6 Å4.6 Å3.9 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.19N/A-0.43
Procheck G-factor e (all dihedral angles)0.06N/A0.35
Verify3D0.340.0243-1.93
ProsaII (-ve)0.470.0487-0.74
MolProbity clashscore6.131.34080.47
General linear model RMSD prediction1.84
Ramachandran Plot Summary from Procheck f
Most favoured regions89.6%
Additionally allowed regions10.2%
Generously allowed regions0.0%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions97.9%
Allowed regions2%
Disallowed regions0.1%


a Analysed for residues 1 to 134
b There are 133 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 5A-80A,86A-119A,129A-133A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-80A,86A-119A,129A-132A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4