Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1700
intra-residue [i = j]477
sequential [| i - j | = 1]441
medium range [1 < | i - j | < 5]170
long range [| i - j | ≥ 5]612
NOE constraints per restrained residue b17.3
Dihedral-angle constraints:125
Total number of restricting constraints b1825
Total number of restricting constraints per restrained residue b18.6
Restricting long-range constraints per restrained residue b6.2
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å15.15
0.2 - 0.5 Å31.05
> 0.5 Å73.25
RMS of distance violation / constraint0.46 Å
Maximum distance violation d6.38 Å
Dihedral angle violations / structure
1 - 10 °4.05
> 10 °4.8
RMS of dihedral angle violation / constraint11.64 °
Maximum dihedral angle violation d111.30 °
RPF scores
RecallPrecisionF-measureDP-score
0.9840.8830.9310.809
RMSD Values
allorderedeSelectedf
All backbone atoms1.1 Å0.6 Å0.6 Å
All heavy atoms1.5 Å1.0 Å1.0 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.44N/A-1.42
Procheck G-factor e (all dihedral angles)-0.04N/A-0.24
Verify3D0.470.02070.16
ProsaII (-ve)0.430.0469-0.91
MolProbity clashscore3.371.42960.95
General linear model RMSD prediction1.51
Ramachandran Plot Summary from Procheck f
Most favoured regions94.9%
Additionally allowed regions3.8%
Generously allowed regions0.2%
Disallowed regions1.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98%
Allowed regions2%
Disallowed regions0%


a Analysed for residues 1 to 98
b There are 98 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 1A-35A,37A-78A,83A-93A
f Residues selected based on: User defined residues

Selected residue ranges: 2A-33A,38A-78A,83A-94A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4