Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1700
intra-residue [i = j]477
sequential [| i - j | = 1]441
medium range [1 < | i - j | < 5]170
long range [| i - j | ≥ 5]612
NOE constraints per restrained residue b17.3
Dihedral-angle constraints:125
Total number of restricting constraints b1825
Total number of restricting constraints per restrained residue b18.6
Restricting long-range constraints per restrained residue b6.2
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å1.8
0.2 - 0.5 Å1.3
> 0.5 Å0.2
RMS of distance violation / constraint0.01 Å
Maximum distance violation d1.00 Å
Dihedral angle violations / structure
1 - 10 °0.25
> 10 °1
RMS of dihedral angle violation / constraint1.52 °
Maximum dihedral angle violation d40.60 °
RPF scores
RecallPrecisionF-measureDP-score
0.9930.8830.9350.824
RMSD Values
allorderedeSelectedf
All backbone atoms0.9 Å0.4 Å0.4 Å
All heavy atoms1.3 Å0.7 Å0.7 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.37N/A-1.14
Procheck G-factor e (all dihedral angles)-0.02N/A-0.12
Verify3D0.490.02310.48
ProsaII (-ve)0.430.0365-0.91
MolProbity clashscore2.091.19501.17
General linear model RMSD prediction1.38
Ramachandran Plot Summary from Procheck f
Most favoured regions95.3%
Additionally allowed regions3.4%
Generously allowed regions1.2%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions97.9%
Allowed regions2.1%
Disallowed regions0%


a Analysed for residues 1 to 98
b There are 98 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 2A-35A,37A-78A,83A-94A
f Residues selected based on: User defined residues

Selected residue ranges: 2A-33A,38A-78A,83A-94A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4