Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2386
intra-residue [i = j]572
sequential [| i - j | = 1]588
medium range [1 < | i - j | < 5]594
long range [| i - j | ≥ 5]632
NOE constraints per restrained residue b23.6
Hydrogen bond constraints:
Total228
long range [| i - j | ≥ 5]32
Dihedral-angle constraints:336
Total number of restricting constraints b2950
Total number of restricting constraints per restrained residue b29.2
Restricting long-range constraints per restrained residue b6.6
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å18.8
0.2 - 0.5 Å29.25
> 0.5 Å56.8
RMS of distance violation / constraint0.32 Å
Maximum distance violation d6.78 Å
Dihedral angle violations / structure
1 - 10 °4.5
> 10 °2.95
RMS of dihedral angle violation / constraint1.88 °
Maximum dihedral angle violation d40.70 °
RPF scores
RecallPrecisionF-measureDP-score
0.9510.8420.8930.765
RMSD Values
allorderedeSelectedf
All backbone atoms3.3 Å1.3 Å1.3 Å
All heavy atoms3.7 Å1.6 Å1.6 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.31N/A1.53
Procheck G-factor e (all dihedral angles)0.42N/A2.48
Verify3D0.420.0176-0.64
ProsaII (-ve)0.860.05600.87
MolProbity clashscore2.761.36291.05
General linear model RMSD prediction2.18
Ramachandran Plot Summary from Procheck f
Most favoured regions98.0%
Additionally allowed regions2.0%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.7%
Allowed regions0.3%
Disallowed regions0%


a Analysed for residues 1 to 226
b There are 101 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 7A-22A,26A-65A,68A-101A,7B-22B,26B-65B,68B-101B
f Residues selected based on: User defined residues

Selected residue ranges: 7A-22A,26A-65A,68A-101A,7B-22B,26B-100B

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4