Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2384
intra-residue [i = j]570
sequential [| i - j | = 1]588
medium range [1 < | i - j | < 5]594
long range [| i - j | ≥ 5]632
NOE constraints per restrained residue b23.6
Hydrogen bond constraints:
Total228
long range [| i - j | ≥ 5]40
Dihedral-angle constraints:336
Total number of restricting constraints b2948
Total number of restricting constraints per restrained residue b29.2
Restricting long-range constraints per restrained residue b6.7
Total structures computedcurrently unknown
Number of structures used18
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å4.67
0.2 - 0.5 Å1.56
> 0.5 Å1
RMS of distance violation / constraint0.02 Å
Maximum distance violation d1.16 Å
Dihedral angle violations / structure
1 - 10 °0
> 10 °0
RMS of dihedral angle violation / constraint0.00 °
Maximum dihedral angle violation d0 °
RPF scores
RecallPrecisionF-measureDP-score
0.9550.8390.8940.77
RMSD Values
allorderedeSelectedf
All backbone atoms2.8 Å0.7 Å0.7 Å
All heavy atoms3.2 Å1.1 Å1.1 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.49N/A2.24
Procheck G-factor e (all dihedral angles)0.52N/A3.08
Verify3D0.420.0229-0.64
ProsaII (-ve)0.880.06160.95
MolProbity clashscore4.111.54130.82
General linear model RMSD prediction1.99
Ramachandran Plot Summary from Procheck f
Most favoured regions99.7%
Additionally allowed regions0.3%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.9%
Allowed regions0.1%
Disallowed regions0%


a Analysed for residues 1 to 226
b There are 101 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 7A-22A,26A-65A,68A-101A,7B-22B,26B-65B,68B-101B
f Residues selected based on: User defined residues

Selected residue ranges: 7A-22A,26A-65A,68A-101A,7B-22B,26B-100B

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4