Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2542
intra-residue [i = j]485
sequential [| i - j | = 1]722
medium range [1 < | i - j | < 5]314
long range [| i - j | ≥ 5]1021
NOE constraints per restrained residue b23.8
Hydrogen bond constraints:
Total56
long range [| i - j | ≥ 5]41
Dihedral-angle constraints:94
Total number of restricting constraints b2692
Total number of restricting constraints per restrained residue b25.2
Restricting long-range constraints per restrained residue b9.9
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å15.3
0.2 - 0.5 Å39.8
> 0.5 Å113.15
RMS of distance violation / constraint0.57 Å
Maximum distance violation d7.86 Å
Dihedral angle violations / structure
1 - 10 °6.9
> 10 °4
RMS of dihedral angle violation / constraint3.78 °
Maximum dihedral angle violation d36.00 °
RPF scores
RecallPrecisionF-measureDP-score
0.9580.9570.9580.872
RMSD Values
allorderedeSelectedf
All backbone atoms7.0 Å0.6 Å0.6 Å
All heavy atoms7.7 Å1.0 Å1.0 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.43N/A-1.38
Procheck G-factor e (all dihedral angles)0.02N/A0.12
Verify3D0.340.0264-1.93
ProsaII (-ve)0.520.0523-0.54
MolProbity clashscore3.951.15590.85
General linear model RMSD prediction1.45
Ramachandran Plot Summary from Procheck f
Most favoured regions86.8%
Additionally allowed regions13.1%
Generously allowed regions0.1%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions97%
Allowed regions2.8%
Disallowed regions0.1%


a Analysed for residues 1 to 114
b There are 107 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 7A-37A,42A-48A,51A-92A
f Residues selected based on: User defined residues

Selected residue ranges: 7A-36A,41A-48A,51A-91A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4