Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2542
intra-residue [i = j]485
sequential [| i - j | = 1]722
medium range [1 < | i - j | < 5]314
long range [| i - j | ≥ 5]1021
NOE constraints per restrained residue b23.8
Hydrogen bond constraints:
Total56
long range [| i - j | ≥ 5]41
Dihedral-angle constraints:94
Total number of restricting constraints b2692
Total number of restricting constraints per restrained residue b25.2
Restricting long-range constraints per restrained residue b9.9
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å1.2
0.2 - 0.5 Å0.1
> 0.5 Å0
RMS of distance violation / constraint0.01 Å
Maximum distance violation d0.34 Å
Dihedral angle violations / structure
1 - 10 °3.35
> 10 °0
RMS of dihedral angle violation / constraint0.38 °
Maximum dihedral angle violation d4.90 °
RPF scores
RecallPrecisionF-measureDP-score
0.9790.9550.9670.905
RMSD Values
allorderedeSelectedf
All backbone atoms7.5 Å0.5 Å0.5 Å
All heavy atoms7.8 Å0.8 Å0.8 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.53N/A-1.77
Procheck G-factor e (all dihedral angles)-0.29N/A-1.71
Verify3D0.330.0228-2.09
ProsaII (-ve)0.440.0498-0.87
MolProbity clashscore14.833.1495-1.02
General linear model RMSD prediction1.46
Ramachandran Plot Summary from Procheck f
Most favoured regions85.4%
Additionally allowed regions14.1%
Generously allowed regions0.5%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions94.9%
Allowed regions4.5%
Disallowed regions0.6%


a Analysed for residues 1 to 114
b There are 107 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 7A-36A,41A-48A,51A-91A
f Residues selected based on: User defined residues

Selected residue ranges: 7A-36A,41A-48A,51A-91A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4