Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1755
intra-residue [i = j]455
sequential [| i - j | = 1]498
medium range [1 < | i - j | < 5]256
long range [| i - j | ≥ 5]546
NOE constraints per restrained residue b20.6
Dihedral-angle constraints:125
Total number of restricting constraints b1880
Total number of restricting constraints per restrained residue b22.1
Restricting long-range constraints per restrained residue b6.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å10.6
0.2 - 0.5 Å19
> 0.5 Å40.95
RMS of distance violation / constraint0.34 Å
Maximum distance violation d4.83 Å
Dihedral angle violations / structure
1 - 10 °4.65
> 10 °0.85
RMS of dihedral angle violation / constraint2.45 °
Maximum dihedral angle violation d45.50 °
RPF scores
RecallPrecisionF-measureDP-score
0.9750.930.9520.805
RMSD Values
allorderedeSelectedf
All backbone atoms1.9 Å0.5 Å0.5 Å
All heavy atoms2.2 Å0.9 Å0.9 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.29N/A-0.83
Procheck G-factor e (all dihedral angles)0.08N/A0.47
Verify3D0.410.0200-0.80
ProsaII (-ve)0.650.04410.00
MolProbity clashscore2.971.16811.02
General linear model RMSD prediction1.25
Ramachandran Plot Summary from Procheck f
Most favoured regions93.1%
Additionally allowed regions6.9%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.5%
Allowed regions0.5%
Disallowed regions0%


a Analysed for residues 1 to 86
b There are 85 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 8A-83A
f Residues selected based on: User defined residues

Selected residue ranges: 8A-82A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4