Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2481
intra-residue [i = j]559
sequential [| i - j | = 1]599
medium range [1 < | i - j | < 5]469
long range [| i - j | ≥ 5]854
NOE constraints per restrained residue b30.6
Hydrogen bond constraints:
Total48
long range [| i - j | ≥ 5]12
Dihedral-angle constraints:84
Total number of restricting constraints b2613
Total number of restricting constraints per restrained residue b32.3
Restricting long-range constraints per restrained residue b10.7
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å4.8
0.2 - 0.5 Å3.25
> 0.5 Å0.45
RMS of distance violation / constraint0.01 Å
Maximum distance violation d0.82 Å
Dihedral angle violations / structure
1 - 10 °0.05
> 10 °0
RMS of dihedral angle violation / constraint0.04 °
Maximum dihedral angle violation d1.40 °
RPF scores
RecallPrecisionF-measureDP-score
0.980.9730.9760.907
RMSD Values
allorderedeSelectedf
All backbone atoms3.9 Å0.3 Å0.3 Å
All heavy atoms4.3 Å0.5 Å0.5 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.07N/A0.59
Procheck G-factor e (all dihedral angles)0.23N/A1.36
Verify3D0.300.0336-2.57
ProsaII (-ve)0.600.0581-0.21
MolProbity clashscore4.302.13180.79
General linear model RMSD prediction0.61
Ramachandran Plot Summary from Procheck f
Most favoured regions96.2%
Additionally allowed regions3.8%
Generously allowed regions0.0%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.5%
Allowed regions0.3%
Disallowed regions0.2%


a Analysed for residues 1 to 91
b There are 81 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 19A-38A,44A-81A
f Residues selected based on: User defined residues

Selected residue ranges: 19A-38A,44A-81A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4