Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2481
intra-residue [i = j]559
sequential [| i - j | = 1]599
medium range [1 < | i - j | < 5]469
long range [| i - j | ≥ 5]854
NOE constraints per restrained residue b30.6
Hydrogen bond constraints:
Total48
long range [| i - j | ≥ 5]12
Dihedral-angle constraints:84
Total number of restricting constraints b2613
Total number of restricting constraints per restrained residue b32.3
Restricting long-range constraints per restrained residue b10.7
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å1.4
0.2 - 0.5 Å0.15
> 0.5 Å0
RMS of distance violation / constraint0.00 Å
Maximum distance violation d0.27 Å
Dihedral angle violations / structure
1 - 10 °0.3
> 10 °0
RMS of dihedral angle violation / constraint0.16 °
Maximum dihedral angle violation d2.60 °
RPF scores
RecallPrecisionF-measureDP-score
0.980.9730.9760.907
RMSD Values
allorderedeSelectedf
All backbone atoms3.8 Å0.4 Å0.4 Å
All heavy atoms4.3 Å0.8 Å0.8 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.20N/A-0.47
Procheck G-factor e (all dihedral angles)-0.06N/A-0.35
Verify3D0.250.0339-3.37
ProsaII (-ve)0.500.0667-0.62
MolProbity clashscore14.602.2559-0.98
General linear model RMSD prediction0.90
Ramachandran Plot Summary from Procheck f
Most favoured regions91.9%
Additionally allowed regions7.9%
Generously allowed regions0.1%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.8%
Allowed regions1%
Disallowed regions0.2%


a Analysed for residues 1 to 91
b There are 81 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 19A-38A,44A-81A
f Residues selected based on: User defined residues

Selected residue ranges: 19A-38A,44A-81A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4