Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total712
intra-residue [i = j]80
sequential [| i - j | = 1]245
medium range [1 < | i - j | < 5]236
long range [| i - j | ≥ 5]151
NOE constraints per restrained residue b9.6
Dihedral-angle constraints:90
Total number of restricting constraints b802
Total number of restricting constraints per restrained residue b10.8
Restricting long-range constraints per restrained residue b2.0
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å1.15
0.2 - 0.5 Å3.35
> 0.5 Å7.65
RMS of distance violation / constraint0.37 Å
Maximum distance violation d6.49 Å
Dihedral angle violations / structure
1 - 10 °1.3
> 10 °1
RMS of dihedral angle violation / constraint2.13 °
Maximum dihedral angle violation d35.40 °
RPF scores
RecallPrecisionF-measureDP-score
0.9150.9170.9160.725
RMSD Values
allorderedeSelectedf
All backbone atoms3.8 Å1.1 Å1.1 Å
All heavy atoms4.3 Å1.5 Å1.5 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.32N/A1.57
Procheck G-factor e (all dihedral angles)0.45N/A2.66
Verify3D0.390.0369-1.12
ProsaII (-ve)0.880.08770.95
MolProbity clashscore3.021.38711.01
General linear model RMSD prediction0.90
Ramachandran Plot Summary from Procheck f
Most favoured regions95.4%
Additionally allowed regions4.6%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.6%
Allowed regions1.3%
Disallowed regions0.1%


a Analysed for residues 1 to 83
b There are 74 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 11A-13A,18A-54A,61A-77A
f Residues selected based on: User defined residues

Selected residue ranges: 11A-13A,18A-54A,62A-77A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4