Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total3491
intra-residue [i = j]668
sequential [| i - j | = 1]916
medium range [1 < | i - j | < 5]808
long range [| i - j | ≥ 5]1099
NOE constraints per restrained residue b20.8
Hydrogen bond constraints:
Total76
long range [| i - j | ≥ 5]16
Dihedral-angle constraints:364
Total number of restricting constraints b3931
Total number of restricting constraints per restrained residue b23.4
Restricting long-range constraints per restrained residue b6.6
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å21.35
0.2 - 0.5 Å45.7
> 0.5 Å99.65
RMS of distance violation / constraint0.67 Å
Maximum distance violation d9.35 Å
Dihedral angle violations / structure
1 - 10 °13.25
> 10 °7.8
RMS of dihedral angle violation / constraint3.48 °
Maximum dihedral angle violation d48.40 °
RPF scores
RecallPrecisionF-measureDP-score
0.920.9610.940.78
RMSD Values
allorderedeSelectedf
All backbone atoms3.7 Å1.2 Å1.2 Å
All heavy atoms4.3 Å1.7 Å1.6 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.08N/A0.00
Procheck G-factor e (all dihedral angles)0.16N/A0.95
Verify3D0.390.0248-1.12
ProsaII (-ve)0.420.0606-0.95
MolProbity clashscore3.941.37110.85
General linear model RMSD prediction2.07
Ramachandran Plot Summary from Procheck f
Most favoured regions93.0%
Additionally allowed regions6.8%
Generously allowed regions0.1%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions97.2%
Allowed regions2.7%
Disallowed regions0.1%


a Analysed for residues 1 to 175
b There are 168 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-17A,22A-73A,76A-97A,100A-102A,119A-131A,133A-159A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-17A,22A-52A,54A-73A,76A-97A,100A-102A,115A-159A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4