Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total3491
intra-residue [i = j]668
sequential [| i - j | = 1]916
medium range [1 < | i - j | < 5]808
long range [| i - j | ≥ 5]1099
NOE constraints per restrained residue b20.8
Hydrogen bond constraints:
Total76
long range [| i - j | ≥ 5]16
Dihedral-angle constraints:364
Total number of restricting constraints b3931
Total number of restricting constraints per restrained residue b23.4
Restricting long-range constraints per restrained residue b6.6
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å5.25
0.2 - 0.5 Å2.85
> 0.5 Å0.55
RMS of distance violation / constraint0.01 Å
Maximum distance violation d0.97 Å
Dihedral angle violations / structure
1 - 10 °2.1
> 10 °0.7
RMS of dihedral angle violation / constraint0.92 °
Maximum dihedral angle violation d32.60 °
RPF scores
RecallPrecisionF-measureDP-score
0.9590.9530.9560.841
RMSD Values
allorderedeSelectedf
All backbone atoms3.6 Å0.9 Å0.9 Å
All heavy atoms4.1 Å1.2 Å1.2 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.05N/A0.51
Procheck G-factor e (all dihedral angles)0.19N/A1.12
Verify3D0.370.0191-1.44
ProsaII (-ve)0.410.0588-0.99
MolProbity clashscore4.800.99170.70
General linear model RMSD prediction1.74
Ramachandran Plot Summary from Procheck f
Most favoured regions93.8%
Additionally allowed regions6.0%
Generously allowed regions0.1%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions97%
Allowed regions2.8%
Disallowed regions0.2%


a Analysed for residues 1 to 175
b There are 168 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-17A,22A-52A,54A-73A,76A-97A,100A-102A,115A-159A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-17A,22A-52A,54A-73A,76A-97A,100A-102A,115A-159A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4