Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1571
intra-residue [i = j]333
sequential [| i - j | = 1]481
medium range [1 < | i - j | < 5]425
long range [| i - j | ≥ 5]332
NOE constraints per restrained residue b14.7
Dihedral-angle constraints:399
Total number of restricting constraints b1970
Total number of restricting constraints per restrained residue b18.4
Restricting long-range constraints per restrained residue b3.1
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å16.2
0.2 - 0.5 Å33.5
> 0.5 Å61.95
RMS of distance violation / constraint0.93 Å
Maximum distance violation d14.48 Å
Dihedral angle violations / structure
1 - 10 °12.55
> 10 °7.4
RMS of dihedral angle violation / constraint7.69 °
Maximum dihedral angle violation d149.20 °
RPF scores
RecallPrecisionF-measureDP-score
0.9320.9490.9410.683
RMSD Values
allorderedeSelectedf
All backbone atoms9.0 Å1.2 Å1.2 Å
All heavy atoms9.3 Å1.6 Å1.6 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.21N/A1.14
Procheck G-factor e (all dihedral angles)0.31N/A1.83
Verify3D0.370.0441-1.44
ProsaII (-ve)0.600.0538-0.21
MolProbity clashscore3.981.47800.84
General linear model RMSD prediction1.55
Ramachandran Plot Summary from Procheck f
Most favoured regions92.2%
Additionally allowed regions7.8%
Generously allowed regions0.1%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions97.9%
Allowed regions2%
Disallowed regions0.1%


a Analysed for residues 1 to 121
b There are 107 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 24A-44A,48A-82A,84A-102A,105A-113A
f Residues selected based on: User defined residues

Selected residue ranges: 24A-44A,48A-82A,84A-102A,105A-113A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4