Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total893
intra-residue [i = j]2
sequential [| i - j | = 1]129
medium range [1 < | i - j | < 5]121
long range [| i - j | ≥ 5]641
NOE constraints per restrained residue b6.4
Hydrogen bond constraints:
Total62
long range [| i - j | ≥ 5]62
Dihedral-angle constraints:210
Total number of restricting constraints b1165
Total number of restricting constraints per restrained residue b8.3
Restricting long-range constraints per restrained residue b5.0
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å1.6
0.2 - 0.5 Å2.05
> 0.5 Å1.9
RMS of distance violation / constraint0.04 Å
Maximum distance violation d1.32 Å
Dihedral angle violations / structure
1 - 10 °1.05
> 10 °0.8
RMS of dihedral angle violation / constraint1.15 °
Maximum dihedral angle violation d28.90 °
RPF scores
RecallPrecisionF-measureDP-score
0.9011570.8828050.8920.834437
RMSD Values
allorderedeSelectedf
All backbone atoms0.9 Å0.5 Å0.5 Å
All heavy atoms1.2 Å0.9 Å0.9 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.36N/A-1.10
Procheck G-factor e (all dihedral angles)-0.00N/A-0.00
Verify3D0.430.0150-0.48
ProsaII (-ve)0.290.0389-1.49
MolProbity clashscore5.261.36780.62
General linear model RMSD prediction1.82
Ramachandran Plot Summary from Procheck f
Most favoured regions90.8%
Additionally allowed regions8.8%
Generously allowed regions0.3%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions97.6%
Allowed regions2.2%
Disallowed regions0.2%


a Analysed for residues 1 to 143
b There are 140 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-16A,22A-30A,33A-67A,71A-139A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-16A,19A-30A,33A-67A,70A-139A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4