Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total893
intra-residue [i = j]2
sequential [| i - j | = 1]129
medium range [1 < | i - j | < 5]121
long range [| i - j | ≥ 5]641
NOE constraints per restrained residue b6.4
Hydrogen bond constraints:
Total62
long range [| i - j | ≥ 5]62
Dihedral-angle constraints:210
Total number of restricting constraints b1165
Total number of restricting constraints per restrained residue b8.3
Restricting long-range constraints per restrained residue b5.0
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å2.1
0.2 - 0.5 Å2.05
> 0.5 Å0
RMS of distance violation / constraint0.02 Å
Maximum distance violation d0.48 Å
Dihedral angle violations / structure
1 - 10 °0.05
> 10 °0
RMS of dihedral angle violation / constraint0.05 °
Maximum dihedral angle violation d1.10 °
RPF scores
RecallPrecisionF-measureDP-score
0.8937960.8830850.8880.818482
RMSD Values
allorderedeSelectedf
All backbone atoms0.9 Å0.7 Å0.7 Å
All heavy atoms1.4 Å1.2 Å1.2 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.67N/A-2.32
Procheck G-factor e (all dihedral angles)-0.48N/A-2.84
Verify3D0.390.0230-1.12
ProsaII (-ve)0.270.0459-1.57
MolProbity clashscore23.822.5367-2.56
General linear model RMSD prediction2.22
Ramachandran Plot Summary from Procheck f
Most favoured regions85.5%
Additionally allowed regions13.7%
Generously allowed regions0.7%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions94.7%
Allowed regions4.7%
Disallowed regions0.6%


a Analysed for residues 1 to 143
b There are 140 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-16A,19A-30A,33A-67A,70A-139A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-16A,19A-30A,33A-67A,70A-139A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4