Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total979
intra-residue [i = j]144
sequential [| i - j | = 1]359
medium range [1 < | i - j | < 5]207
long range [| i - j | ≥ 5]269
NOE constraints per restrained residue b12.9
Hydrogen bond constraints:
Total31
long range [| i - j | ≥ 5]19
Dihedral-angle constraints:81
Total number of restricting constraints b1091
Total number of restricting constraints per restrained residue b14.4
Restricting long-range constraints per restrained residue b3.8
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å4.95
0.2 - 0.5 Å1.6
> 0.5 Å1.65
RMS of distance violation / constraint0.04 Å
Maximum distance violation d1.00 Å
Dihedral angle violations / structure
1 - 10 °0.35
> 10 °0
RMS of dihedral angle violation / constraint0.31 °
Maximum dihedral angle violation d7.80 °
RPF scores
RecallPrecisionF-measureDP-score
0.9880.9260.9560.853
RMSD Values
allorderedeSelectedf
All backbone atoms4.8 Å0.7 Å0.7 Å
All heavy atoms5.5 Å1.0 Å1.0 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.20N/A-0.47
Procheck G-factor e (all dihedral angles)0.07N/A0.41
Verify3D0.370.0439-1.44
ProsaII (-ve)0.510.0851-0.58
MolProbity clashscore4.671.83150.72
General linear model RMSD prediction0.92
Ramachandran Plot Summary from Procheck f
Most favoured regions95.0%
Additionally allowed regions4.8%
Generously allowed regions0.1%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.5%
Allowed regions1.3%
Disallowed regions0.3%


a Analysed for residues 1 to 78
b There are 76 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 1A-13A,18A-29A,32A-63A
f Residues selected based on: User defined residues

Selected residue ranges: 1A-13A,18A-29A,32A-63A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4