Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total445
intra-residue [i = j]1
sequential [| i - j | = 1]140
medium range [1 < | i - j | < 5]89
long range [| i - j | ≥ 5]215
NOE constraints per restrained residue b9.7
Hydrogen bond constraints:
Total20
long range [| i - j | ≥ 5]17
Dihedral-angle constraints:43
Total number of restricting constraints b508
Total number of restricting constraints per restrained residue b11.0
Restricting long-range constraints per restrained residue b5.0
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å3.65
0.2 - 0.5 Å1.8
> 0.5 Å1.1
RMS of distance violation / constraint0.04 Å
Maximum distance violation d0.92 Å
Dihedral angle violations / structure
1 - 10 °0.05
> 10 °1.65
RMS of dihedral angle violation / constraint6.59 °
Maximum dihedral angle violation d64.70 °
RMSD Values
allorderedeSelectedf
All backbone atoms1.2 Å0.5 Å0.5 Å
All heavy atoms1.9 Å0.9 Å0.9 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.41N/A-1.30
Procheck G-factor e (all dihedral angles)-0.03N/A-0.18
Verify3D0.240.0363-3.53
ProsaII (-ve)0.420.0533-0.95
MolProbity clashscore5.072.66420.66
Ramachandran Plot Summary from Procheck f
Most favoured regions91.1%
Additionally allowed regions8.5%
Generously allowed regions0.3%
Disallowed regions0.1%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions95.7%
Allowed regions3.9%
Disallowed regions0.4%


a Analysed for residues 1 to 50
b There are 46 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-14A,18A-46A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-14A,17A-46A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4