Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1362
intra-residue [i = j]260
sequential [| i - j | = 1]478
medium range [1 < | i - j | < 5]144
long range [| i - j | ≥ 5]480
NOE constraints per restrained residue b9.1
Dihedral-angle constraints:656
Total number of restricting constraints b2018
Total number of restricting constraints per restrained residue b13.5
Restricting long-range constraints per restrained residue b3.2
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å10.35
0.2 - 0.5 Å17.45
> 0.5 Å41.4
RMS of distance violation / constraint0.49 Å
Maximum distance violation d9.11 Å
Dihedral angle violations / structure
1 - 10 °17.95
> 10 °20.9
RMS of dihedral angle violation / constraint7.32 °
Maximum dihedral angle violation d111.50 °
RPF scores
RecallPrecisionF-measureDP-score
0.8930.9430.9170.62
RMSD Values
allorderedeSelectedf
All backbone atoms5.6 Å3.3 Å3.4 Å
All heavy atoms6.2 Å3.7 Å3.7 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.33N/A-0.98
Procheck G-factor e (all dihedral angles)-0.05N/A-0.30
Verify3D0.280.0368-2.89
ProsaII (-ve)0.140.0880-2.11
MolProbity clashscore5.781.57610.53
General linear model RMSD prediction2.65
Ramachandran Plot Summary from Procheck f
Most favoured regions90.3%
Additionally allowed regions7.7%
Generously allowed regions2.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions96.3%
Allowed regions2.4%
Disallowed regions1.3%


a Analysed for residues 1 to 155
b There are 149 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-23A,65A-70A,73A-78A,90A-97A,102A-109A,116A-124A,135A-146A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-23A,63A-70A,73A-78A,90A-97A,100A-112A,116A-124A,135A-146A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4