Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1362
intra-residue [i = j]260
sequential [| i - j | = 1]478
medium range [1 < | i - j | < 5]144
long range [| i - j | ≥ 5]480
NOE constraints per restrained residue b9.1
Dihedral-angle constraints:656
Total number of restricting constraints b2018
Total number of restricting constraints per restrained residue b13.5
Restricting long-range constraints per restrained residue b3.2
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å8.3
0.2 - 0.5 Å2.15
> 0.5 Å0
RMS of distance violation / constraint0.02 Å
Maximum distance violation d0.43 Å
Dihedral angle violations / structure
1 - 10 °22.05
> 10 °0
RMS of dihedral angle violation / constraint0.45 °
Maximum dihedral angle violation d9.60 °
RPF scores
RecallPrecisionF-measureDP-score
0.9080.930.9190.629
RMSD Values
allorderedeSelectedf
All backbone atoms5.2 Å3.0 Å3.0 Å
All heavy atoms5.9 Å3.4 Å3.4 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.36N/A-1.10
Procheck G-factor e (all dihedral angles)-0.47N/A-2.78
Verify3D0.180.0299-4.49
ProsaII (-ve)0.020.0442-2.61
MolProbity clashscore35.103.8464-4.50
General linear model RMSD prediction2.73
Ramachandran Plot Summary from Procheck f
Most favoured regions93.5%
Additionally allowed regions5.0%
Generously allowed regions1.5%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions94.9%
Allowed regions3.4%
Disallowed regions1.6%


a Analysed for residues 1 to 155
b There are 149 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-23A,63A-70A,73A-78A,90A-97A,100A-112A,116A-124A,135A-146A
f Residues selected based on: User defined residues

Selected residue ranges: 4A-23A,63A-70A,73A-78A,90A-97A,100A-112A,116A-124A,135A-146A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4