Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1769
intra-residue [i = j]500
sequential [| i - j | = 1]412
medium range [1 < | i - j | < 5]87
long range [| i - j | ≥ 5]770
NOE constraints per restrained residue b28.1
Hydrogen bond constraints:
Total80
long range [| i - j | ≥ 5]72
Dihedral-angle constraints:152
Total number of restricting constraints b2001
Total number of restricting constraints per restrained residue b31.8
Restricting long-range constraints per restrained residue b13.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å19.05
0.2 - 0.5 Å37.6
> 0.5 Å95.45
RMS of distance violation / constraint0.35 Å
Maximum distance violation d4.05 Å
Dihedral angle violations / structure
1 - 10 °12.8
> 10 °9.2
RMS of dihedral angle violation / constraint5.63 °
Maximum dihedral angle violation d63.80 °
RMSD Values
allorderedeSelectedf
All backbone atoms3.1 Å0.5 Å0.4 Å
All heavy atoms3.9 Å0.9 Å0.8 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.71N/A-2.48
Procheck G-factor e (all dihedral angles)-0.19N/A-1.12
Verify3D0.370.0404-1.44
ProsaII (-ve)0.340.1060-1.28
MolProbity clashscore5.752.29880.54
Ramachandran Plot Summary from Procheck f
Most favoured regions87.3%
Additionally allowed regions12.7%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.8%
Allowed regions1.2%
Disallowed regions0%


a Analysed for residues 1 to 152
b There are 63 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 5A-27A,30A-40A,50A-62A,5B-27B,30B-40B,50B-62B
f Residues selected based on: User defined residues

Selected residue ranges: 5A-27A,30A-40A,50A-62A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4