Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1769
intra-residue [i = j]500
sequential [| i - j | = 1]412
medium range [1 < | i - j | < 5]87
long range [| i - j | ≥ 5]770
NOE constraints per restrained residue b28.1
Hydrogen bond constraints:
Total80
long range [| i - j | ≥ 5]72
Dihedral-angle constraints:152
Total number of restricting constraints b2001
Total number of restricting constraints per restrained residue b31.8
Restricting long-range constraints per restrained residue b13.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å4.9
0.2 - 0.5 Å0.6
> 0.5 Å0.5
RMS of distance violation / constraint0.02 Å
Maximum distance violation d0.84 Å
Dihedral angle violations / structure
1 - 10 °3
> 10 °2.4
RMS of dihedral angle violation / constraint2.30 °
Maximum dihedral angle violation d43.60 °
RMSD Values
allorderedeSelectedf
All backbone atoms2.9 Å0.4 Å0.3 Å
All heavy atoms3.7 Å0.7 Å0.6 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.60N/A-2.05
Procheck G-factor e (all dihedral angles)-0.17N/A-1.01
Verify3D0.340.0348-1.93
ProsaII (-ve)0.340.1054-1.28
MolProbity clashscore5.752.44620.54
Ramachandran Plot Summary from Procheck f
Most favoured regions86.7%
Additionally allowed regions13.3%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.9%
Allowed regions1.1%
Disallowed regions0%


a Analysed for residues 1 to 152
b There are 63 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 5A-27A,30A-40A,50A-62A,5B-27B,30B-40B,50B-62B
f Residues selected based on: User defined residues

Selected residue ranges: 5A-27A,30A-40A,50A-62A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4