Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total793
intra-residue [i = j]157
sequential [| i - j | = 1]225
medium range [1 < | i - j | < 5]75
long range [| i - j | ≥ 5]336
NOE constraints per restrained residue b9.2
Hydrogen bond constraints:
Total48
long range [| i - j | ≥ 5]46
Dihedral-angle constraints:66
Total number of restricting constraints b907
Total number of restricting constraints per restrained residue b10.5
Restricting long-range constraints per restrained residue b4.4
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å3.35
0.2 - 0.5 Å2
> 0.5 Å0.35
RMS of distance violation / constraint0.03 Å
Maximum distance violation d1.22 Å
Dihedral angle violations / structure
1 - 10 °0
> 10 °0
RMS of dihedral angle violation / constraint0.00 °
Maximum dihedral angle violation d0 °
RPF scores
RecallPrecisionF-measureDP-score
0.9560.8610.9060.838
RMSD Values
allorderedeSelectedf
All backbone atoms2.2 Å0.5 Å0.5 Å
All heavy atoms2.5 Å0.9 Å0.9 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.44N/A-1.42
Procheck G-factor e (all dihedral angles)-0.05N/A-0.30
Verify3D0.380.0395-1.28
ProsaII (-ve)0.520.0556-0.54
MolProbity clashscore4.051.34070.83
General linear model RMSD prediction1.39
Ramachandran Plot Summary from Procheck f
Most favoured regions94.0%
Additionally allowed regions6.0%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.4%
Allowed regions1.1%
Disallowed regions0.5%


a Analysed for residues 1 to 96
b There are 86 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 9A-17A,24A-42A,45A-55A,59A-72A,75A-79A,81A-91A
f Residues selected based on: User defined residues

Selected residue ranges: 9A-17A,24A-42A,45A-55A,59A-72A,75A-91A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4