Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total2530
intra-residue [i = j]432
sequential [| i - j | = 1]592
medium range [1 < | i - j | < 5]536
long range [| i - j | ≥ 5]970
NOE constraints per restrained residue b30.9
Dihedral-angle constraints:116
Total number of restricting constraints b2646
Total number of restricting constraints per restrained residue b32.3
Restricting long-range constraints per restrained residue b11.8
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å20.5
0.2 - 0.5 Å10.75
> 0.5 Å2.35
RMS of distance violation / constraint0.04 Å
Maximum distance violation d1.09 Å
Dihedral angle violations / structure
1 - 10 °0.25
> 10 °0
RMS of dihedral angle violation / constraint0.09 °
Maximum dihedral angle violation d2.40 °
RPF scores
RecallPrecisionF-measureDP-score
0.9110.8440.8760.824
RMSD Values
allorderedeSelectedf
All backbone atoms1.6 Å0.5 Å0.4 Å
All heavy atoms2.0 Å0.6 Å0.6 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.02N/A0.39
Procheck G-factor e (all dihedral angles)0.02N/A0.12
Verify3D0.450.0300-0.16
ProsaII (-ve)0.700.04260.21
MolProbity clashscore9.131.4737-0.04
General linear model RMSD prediction1.85
Ramachandran Plot Summary from Procheck f
Most favoured regions96.1%
Additionally allowed regions3.8%
Generously allowed regions0.1%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.6%
Allowed regions0.4%
Disallowed regions0%


a Analysed for residues 1 to 186
b There are 82 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 4A-15A,18A-28A,31A-41A,43A-82A,4B-15B,18B-28B,31B-41B,43B-82B
f Residues selected based on: User defined residues

Selected residue ranges: 4A-15A,18A-28A,31A-41A,43A-82A,3B-15B,18B-41B,43B-77B

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4