Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1106
intra-residue [i = j]211
sequential [| i - j | = 1]406
medium range [1 < | i - j | < 5]105
long range [| i - j | ≥ 5]384
NOE constraints per restrained residue b6.9
Hydrogen bond constraints:
Total44
long range [| i - j | ≥ 5]40
Dihedral-angle constraints:687
Total number of restricting constraints b1837
Total number of restricting constraints per restrained residue b11.4
Restricting long-range constraints per restrained residue b2.6
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å11.5
0.2 - 0.5 Å15.9
> 0.5 Å29.65
RMS of distance violation / constraint0.60 Å
Maximum distance violation d10.55 Å
Dihedral angle violations / structure
1 - 10 °19.5
> 10 °22.75
RMS of dihedral angle violation / constraint6.45 °
Maximum dihedral angle violation d137.90 °
RPF scores
RecallPrecisionF-measureDP-score
0.9570.9450.9510.71
RMSD Values
allorderedeSelectedf
All backbone atoms3.8 Å1.6 Å1.7 Å
All heavy atoms4.4 Å2.0 Å2.1 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.29N/A-0.83
Procheck G-factor e (all dihedral angles)-0.06N/A-0.35
Verify3D0.430.0235-0.48
ProsaII (-ve)0.400.0556-1.03
MolProbity clashscore5.931.53890.51
General linear model RMSD prediction2.42
Ramachandran Plot Summary from Procheck f
Most favoured regions92.3%
Additionally allowed regions7.1%
Generously allowed regions0.0%
Disallowed regions0.6%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.1%
Allowed regions1.9%
Disallowed regions0%


a Analysed for residues 1 to 166
b There are 161 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 8A-26A,31A-40A,44A-62A,70A-72A,75A-78A,81A-83A,87A-94A,96A-106A,109A-125A,130A-134A,146A-153A
f Residues selected based on: User defined residues

Selected residue ranges: 8A-27A,31A-40A,44A-62A,68A-73A,75A-78A,81A-83A,87A-106A,109A-126A,130A-134A,138A-140A,146A-153A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4