Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1106
intra-residue [i = j]211
sequential [| i - j | = 1]406
medium range [1 < | i - j | < 5]105
long range [| i - j | ≥ 5]384
NOE constraints per restrained residue b6.9
Hydrogen bond constraints:
Total44
long range [| i - j | ≥ 5]40
Dihedral-angle constraints:687
Total number of restricting constraints b1837
Total number of restricting constraints per restrained residue b11.4
Restricting long-range constraints per restrained residue b2.6
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å7.5
0.2 - 0.5 Å3.65
> 0.5 Å0.3
RMS of distance violation / constraint0.02 Å
Maximum distance violation d0.71 Å
Dihedral angle violations / structure
1 - 10 °5.65
> 10 °4.2
RMS of dihedral angle violation / constraint1.43 °
Maximum dihedral angle violation d32.70 °
RPF scores
RecallPrecisionF-measureDP-score
0.9680.9370.9530.722
RMSD Values
allorderedeSelectedf
All backbone atoms3.7 Å1.4 Å1.5 Å
All heavy atoms4.3 Å1.7 Å1.8 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.11N/A-0.12
Procheck G-factor e (all dihedral angles)0.05N/A0.30
Verify3D0.420.0293-0.64
ProsaII (-ve)0.390.0495-1.08
MolProbity clashscore6.701.56150.38
General linear model RMSD prediction2.21
Ramachandran Plot Summary from Procheck f
Most favoured regions96.5%
Additionally allowed regions2.9%
Generously allowed regions0.0%
Disallowed regions0.6%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.9%
Allowed regions1%
Disallowed regions0%


a Analysed for residues 1 to 166
b There are 161 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 8A-27A,30A-40A,44A-62A,68A-73A,75A-78A,81A-83A,87A-106A,109A-126A,130A-134A,146A-153A
f Residues selected based on: User defined residues

Selected residue ranges: 8A-27A,31A-40A,44A-62A,68A-73A,75A-78A,81A-83A,87A-106A,109A-126A,130A-134A,138A-140A,146A-153A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4