Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1384
intra-residue [i = j]0
sequential [| i - j | = 1]220
medium range [1 < | i - j | < 5]774
long range [| i - j | ≥ 5]390
NOE constraints per restrained residue b21.0
Hydrogen bond constraints:
Total124
long range [| i - j | ≥ 5]0
Dihedral-angle constraints:212
Total number of restricting constraints b1720
Total number of restricting constraints per restrained residue b26.1
Restricting long-range constraints per restrained residue b5.9
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å3
0.2 - 0.5 Å3.1
> 0.5 Å0.1
RMS of distance violation / constraint0.01 Å
Maximum distance violation d0.66 Å
Dihedral angle violations / structure
1 - 10 °0
> 10 °0
RMS of dihedral angle violation / constraint0.00 °
Maximum dihedral angle violation d0 °
RPF scores
RecallPrecisionF-measureDP-score
0.9180.950.9340.774
RMSD Values
allorderedeSelectedf
All backbone atoms2.7 Å0.5 Å0.5 Å
All heavy atoms3.1 Å0.7 Å0.7 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)0.63N/A2.79
Procheck G-factor e (all dihedral angles)0.66N/A3.90
Verify3D0.380.0286-1.28
ProsaII (-ve)0.970.08231.32
MolProbity clashscore2.261.44571.14
General linear model RMSD prediction1.16
Ramachandran Plot Summary from Procheck f
Most favoured regions99.1%
Additionally allowed regions0.9%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.4%
Allowed regions0.6%
Disallowed regions0%


a Analysed for residues 1 to 162
b There are 66 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 8A-69A,8B-69B
f Residues selected based on: User defined residues

Selected residue ranges: 8A-69A,8B-69B

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4