Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1200
intra-residue [i = j]221
sequential [| i - j | = 1]360
medium range [1 < | i - j | < 5]244
long range [| i - j | ≥ 5]375
NOE constraints per restrained residue b10.5
Hydrogen bond constraints:
Total66
long range [| i - j | ≥ 5]38
Dihedral-angle constraints:108
Total number of restricting constraints b1374
Total number of restricting constraints per restrained residue b12.1
Restricting long-range constraints per restrained residue b3.6
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å10.1
0.2 - 0.5 Å20.2
> 0.5 Å57.4
RMS of distance violation / constraint0.53 Å
Maximum distance violation d7.28 Å
Dihedral angle violations / structure
1 - 10 °1.85
> 10 °1.2
RMS of dihedral angle violation / constraint2.06 °
Maximum dihedral angle violation d30.90 °
RPF scores
RecallPrecisionF-measureDP-score
0.930.8590.8930.882
RMSD Values
allorderedeSelectedf
All backbone atoms1.6 Å1.0 Å1.0 Å
All heavy atoms2.0 Å1.4 Å1.4 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.03N/A0.20
Procheck G-factor e (all dihedral angles)0.21N/A1.24
Verify3D0.420.0285-0.64
ProsaII (-ve)0.530.0859-0.50
MolProbity clashscore5.711.68840.55
General linear model RMSD prediction1.06
Ramachandran Plot Summary from Procheck f
Most favoured regions96.0%
Additionally allowed regions4.0%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions99.6%
Allowed regions0.4%
Disallowed regions0%


a Analysed for residues 1 to 116
b There are 114 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 3A-22A,25A-31A,40A-49A,56A-62A,69A-74A,80A-98A,109A-112A
f Residues selected based on: User defined residues

Selected residue ranges: 3A-22A,26A-31A,40A-49A,56A-62A,69A-73A,80A-96A,109A-112A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4