Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1200
intra-residue [i = j]221
sequential [| i - j | = 1]360
medium range [1 < | i - j | < 5]244
long range [| i - j | ≥ 5]375
NOE constraints per restrained residue b10.5
Hydrogen bond constraints:
Total66
long range [| i - j | ≥ 5]38
Dihedral-angle constraints:108
Total number of restricting constraints b1374
Total number of restricting constraints per restrained residue b12.1
Restricting long-range constraints per restrained residue b3.6
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å0.2
0.2 - 0.5 Å0
> 0.5 Å0
RMS of distance violation / constraint0.00 Å
Maximum distance violation d0.17 Å
Dihedral angle violations / structure
1 - 10 °0.1
> 10 °0
RMS of dihedral angle violation / constraint0.07 °
Maximum dihedral angle violation d2.10 °
RPF scores
RecallPrecisionF-measureDP-score
0.9770.8810.9260.938
RMSD Values
allorderedeSelectedf
All backbone atoms1.3 Å0.9 Å0.9 Å
All heavy atoms1.8 Å1.3 Å1.3 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.22N/A-0.55
Procheck G-factor e (all dihedral angles)-0.13N/A-0.77
Verify3D0.360.0260-1.61
ProsaII (-ve)0.490.0525-0.66
MolProbity clashscore19.993.9024-1.90
General linear model RMSD prediction1.09
Ramachandran Plot Summary from Procheck f
Most favoured regions91.8%
Additionally allowed regions8.2%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions96.6%
Allowed regions3.4%
Disallowed regions0%


a Analysed for residues 1 to 116
b There are 114 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 3A-22A,26A-31A,40A-49A,56A-62A,69A-73A,80A-96A,109A-112A
f Residues selected based on: User defined residues

Selected residue ranges: 3A-22A,26A-31A,40A-49A,56A-62A,69A-73A,80A-96A,109A-112A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4