Summary of conformationally-restricting constraints and structure quality factors



Analyses performed for user defined residues.


Summary of conformationally-restricting experimental constraints a
NOE-based distance constraints:
Total1059
intra-residue [i = j]164
sequential [| i - j | = 1]237
medium range [1 < | i - j | < 5]200
long range [| i - j | ≥ 5]458
NOE constraints per restrained residue b8.5
Hydrogen bond constraints:
Total66
long range [| i - j | ≥ 5]36
Dihedral-angle constraints:111
Total number of restricting constraints b1236
Total number of restricting constraints per restrained residue b10.0
Restricting long-range constraints per restrained residue b4.0
Total structures computedcurrently unknown
Number of structures used20
Residual constraint violations a,c
Distance violations / structure
0.1 - 0.2 Å6.85
0.2 - 0.5 Å11.55
> 0.5 Å25.35
RMS of distance violation / constraint0.31 Å
Maximum distance violation d94.99 Å
Dihedral angle violations / structure
1 - 10 °2.35
> 10 °3.6
RMS of dihedral angle violation / constraint5.67 °
Maximum dihedral angle violation d65.10 °
RMSD Values
allorderedeSelectedf
All backbone atoms5.2 Å0.8 Å0.9 Å
All heavy atoms5.8 Å1.2 Å1.3 Å
Structure Quality Factors - overall statistics
Mean scoreSDZ-score g
Procheck G-factor e (phi / psi only)-0.22N/A-0.55
Procheck G-factor e (all dihedral angles)0.04N/A0.24
Verify3D0.410.0221-0.80
ProsaII (-ve)0.420.0686-0.95
MolProbity clashscore4.001.34930.84
Ramachandran Plot Summary from Procheck f
Most favoured regions93.4%
Additionally allowed regions6.6%
Generously allowed regions0.0%
Disallowed regions0.0%
Ramachandran Plot Statistics from Richardson's lab
Most favoured regions98.8%
Allowed regions1.1%
Disallowed regions0.1%


a Analysed for residues 1 to 158
b There are 124 residues with conformationally restricting constraints
c Calculated for all constraints for the given residues, using sum over r^-6
d Largest constraint violation among all the reported structures
e Residues with sum of phi and psi order parameters > 1.8

Ordered residue ranges: 14A-16A,21A-24A,27A-37A,40A-44A,46A-63A,89A-95A,100A-146A
f Residues selected based on: User defined residues

Selected residue ranges: 13A-16A,21A-24A,27A-37A,40A-63A,89A-95A,100A-145A

g With respect to mean and standard deviation for for a set of 252 X-ray structures < 500 residues, of resolution <= 1.80 Å, R-factor <= 0.25 and R-free <= 0.28; a positive value indicates a 'better' score

Generated using PSVS 1.4